bFGF /FGF2 /FGF basic Molecule
FGF basic Molecule Synonym Name
FGF basic Protein Products
|Cat No||Species||Product Description|
|BFF-H4117||Human||Human FGF basic Protein, Tag Free|
|BFF-H8117||Human||Biotinylated Human FGF basic, epitope tag free, ultra sensitivity (primary amine labeling)|
FGF basic Molecule Background
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins. It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.
- (1) Coulier, F. et al.,1997, J. Mol. Evol. 44:43.
- (2) Pereira RC, et al., 2000, Endocrinology 141 (12): 4558–63.
- (3) Liu Y, et al., 2006, Biochem Biophys Res Commun 346 (1): 131–9.