Maltose Binding Protein Molecule Synonym Name
Maltose Binding Protein,MBP,MMBP
Maltose Binding Protein Protein Products
|Cat No||Species||Product Description|
|MBP-O5112||N/A||Recombinant Maltose Binding Protein|
Maltose Binding Protein Molecule Background
Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has an approximate molecular mass of 42.5 kDa. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.
- (1) Duplay P., et al., 1984, J. Biol. Chem. 259:10606-10613.
- (2) Quiocho F.A., et al., 1997, Structure 5:997-1015.