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Cell Culture Products --->CDPro CHO-3 Medium(1X), Liquid

CDPro CHO-3 Medium(1X), Liquid  


BPM CDPro CHO -3 Medium is specifically designed and optimized for recombinant CHO cells (CHO-K1, DG44, DXB11, CHO-S, GS CHO) for easy adaptation without compromising on room of further process development. It is an idea choice when customer have established cell line using serum contained medium and want shift to a chemically defined culture environment for further process development. CDPro CHO-3 medium is suitable for cell culture process of pilot and commercial scale production of antibody/protein. CDPro CHO-3 is an animal origin-free, chemically defined medium that contains no proteins, polypeptides, hydrolysates, or components of unknown composition.

Product Features:
  • Designed for recombinant CHO cell
  • Well-balanced medium for easy adaptation and process development needs
  • Low osmolality characteristics facilitate fed batch process development and optimization
  • Protein free, animal component free, chemically define and regulatory friendly
  • Extensive support by cell culture expert
Concentrated Cell Line Form Serum Endotoxin Glutamine Culture Type Phenol Red
1X CHO Liquid Serum Free Low No Suspension Culture No
Product Classification Buffer system Regulatory Statement Shelf Life
Protein-Free, Chemically Defined;Serum-Free,   Animal Origin-Free Sodium Bicarbonate  For Research and Further Cell Culture Manufacturing Use Only.  18 months
Catalog # Name of Product Product Size Storage Shipping
CM-1123-11 CDPro CHO-3 Medium 500mL at +4℃, in dark RT
CM-1123-12 CDPro CHO-3 Medium 1000mL at +4℃, in dark RT
CM-1123-13 CDPro CHO-3 Medium 10L at +4℃, in dark RT
Precaution:
  • CDPro CHO-3 requires supplementation of L-glutamine or Ala-Gln dipeptide prior to use.
  • CDPro CHO-3 is made without hypoxanthine and thymidine to support DHFR amplified systems.For other applications, HT supplementation is required prior to use.
  • L-glutamine level must be considered in fed batch or perfusion model or your cells are sensitive to ammonia which is more likely generated in presence of  high concentration of glutamine.
  • Formulated without phenol red to avoid estrogen-like effects of phenol red.
Cell Culture Conditions:

Standard cell culture conditions of growing CHO cells in CDPro CHO-3 medium are 36 to 38°C in a humidified air of 5-8% CO2. Under aseptic and cultivation conditions, cell culture vessel can be shaker flask, spinner flask and Wave or any other stirred bioreactors. Shaker flask volume can be from 125mL to 1000mL with approximate 20% to 30% working volume in suspension, and an orbital shaker platform rotating at 115 to 150 rpm is recommended. Loosen caps of flasks or use with vented caps for gas exchange is required for high density cell growth. Avoid overexposure of cultures to light.

Recommended Products:
Catalog # Product Name Descriptions
CM-1121 CDPro CHO-1 Medium Specially designed for transfected CHO cells
CM-1122 CDPro CHO-2 Medium Specially designed for CHO-K1,CHO-S and GS CHO
CM-1124 PFPro CHO-1 Medium Specially designed for DHFR deficient CHO cells
CM-1125 PFPro CHO-2 Medium Easy adaptation CHO medium(coming soon)
CF-1120 CD Feed CHO MAX-1 Concentrated supplement for fed batch process
CF-1121 CD Feed CHO MAX-2 Concentrated supplement for fed batch process