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Biological CRO Services --->Mammalian Cell RPP Services

Mammalian Cell RPP Services  


Mammalian cells have great advantages over other host systems for the production of recombinant proteins, antibodies, and viral proteins, in terms of their ability to produce fully functional proteins that fold properly with complex N-linked and O-linked glycosylation. This comprehensive post-translational modification machinery enables the production of functional proteins for your research needs. Compared with stable cell lines, CHO or 293 transient expression has shorter process time, lower cost, and higher quality.

ACRObiosystems' Rapid Protein Production (RPP) services are based on CHO and 293 transient gene expression. We can deliver to you product on the order of grams in a short time frame using our proprietary platform.
* We offer a risk-free trial for customers who use the request form to send in their DNA sequences. Just send it to us, and we will take care of the rest!

Service Specification

Service Content Approaches  Deliverables Timeline

Gene synthesizing
&Plasmid construction

  • In-house developed codon optimization
  • Gene synthesizing and verification
  • Clone to proprietary mammalian expression vector
  • Gene sequencing report
  • Constructed plasmid
20 days
Plasmid preparation
  • E.coli fermentation
  • Large quantity of plasmid preparation using proprietary technology 
  • > 1mg low endotoxin plasmid DNA
2 days
Pilot evaluation of transient expression and purification
  • Multiple in-house developed chemically defined media/feeds platform screening
  • High throughput purification process screening via DoE
  • Tag free or removal considerations
  • Expression level
  • Purification process data
4-6 days
Process scale up and production
  • Seeds train expansion, bioreactor run and clarification after harvest
  • Chromatography column purification
  • Analytical assay
  • Milligram to gram purified proteins with quality data
  • Documentation
8-10 days

Overlapped timeline: 2-3 weeks * Timeline of service is adjusted based on clients' requirements;

Quotations and Ordering

You can place the order online and make the payment with credit card or Paypal. Also you can place the order off-line by phone, email, or fax with a formal PO (Purchase Order) or credit card. We recommend that you submit your service requests and orders online. 

For questions and quotation requests, please submit your on-line request. Our customer specialist will get back to you within 24 hours from Monday through Friday. However, you can also contact us by email, phone, or fax.

Email: order@acrobiosystems.com
Phone: +1 800-810-0816(US)   +44 208-123-6158(EU)   +86 400-685-2052(AP)
Fax: +1 888-377-6111