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Kits --->Inhibitor Screening Kits

Inhibitor Screening Kits  


Background

Immune checkpoint pathway is a focal point of today’s cancer research. PD-1 is one of the best characterized checkpoint proteins. The binding between PD-1 and its ligand PD-L1 suppresses T-cell activation and allows cancer cells to escape from body’s immune surveillance. Therefore, neutralizing PD-1 pathway activity has been considered a promising strategy by many oncologists.
       This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of new PD-1 pathway inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized human PD-L1 and in-house developed biotinylated PD-1 protein. This product is uniquely suitable for rapid high-throughput screening of putative PD-1 and PD-L1 inhibitors.

Assay Principle

Your experiment will include 4 simple steps: a) Coat the plate with human PD-L1, b) add your molecule of interest to the reactions, c) add biotinylated human PD-1 to bind with PD-L1, and d) add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate to measure the binding activities. The ability of your compound to inhibit PD-1 : PD-L1 binding will be determined by comparing OD readings among different experimental groups.

Immobilized human PD-L1 protein (Catalog# A001-214) at 2 μg/mL (100 μL/well) can bind biotinylated human PD-1 (Catalog# A002-214) with a linear range of 0.038 - 0.6 μg/mL when detected by Streptavidin-HRP (Catalog# A003-214). Inhibition of PD-1-PD-L1 binding by Anti-PD-1 neutralizing antibody (Catalog # PD1-NA001) measured using the PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair (Catalog # EP-101).

Product List

Cat. No. Product Description Size Price
EP-101 PD-1[Biotin]:PD-L1 Inhibitor Screening Pair 96 tests $600
EP-101 PD-1[Biotin]:PD-L1 Inhibitor Screening Pair 480 tests $2,150

Fast and Easy-to-Use

By employing the pre-labeled PD-1 protein, we eliminate the often time-consuming labeling process, and greatly simplify the experimental procedures.

Authentic Human Protein Interaction

Both biotinylated PD-1 and PD-L1 proteins are expressed in the HEK293 cells. The use of human cells as expression host confers authentic post-translational modifications essential for their binding activities.

Anti-PD1 Neutralizing Antibody Included

We include anti-PD1 neutralizing mAb as a standard positive control. This would allow the users to validate their experiments.

High Throughput and Multiple Sizes Available

We offer both 96 tests and 480 tests as standard package sizes, which enables simultaneous analyses of a large amount of inhibitors.

High Detection Sensitivity and Reproducibility

Our biotin labeling platform produces biotinylated proteins with high detection sensitivity and minimal lot-to-lot variation. These features ensure that the assay kit has desired detection sensitivity and the results are reproducible.

Products under Development

PD-1[Biotinylated] : PD-L2CTLA4 [Biotinylated] : B7-1 CTLA4 [Biotinylated] : B7-2