Browse Products By Molecule:

Products --> ActiveMax® Cytokines

ActiveMax® Recombinant Human PDGF-BB  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
PDB-H4219-50ug
    $350.00
PDB-H4219-20ug
    $198.00
In-stock
Place Purchase Orders


Synonym

PDGFBB,PDGFB,FLJ12858,PDGF2,SIS,SSV,c-sis

Source

ActiveMax® Human PDGF-BB (PDB-H4219) is expressed from human 293 cells (HEK293). It contains AA Ser 82 - Thr 190 (Accession # AAH29822).

 

Molecular Characterization

PDGF-B(Ser 82 - Thr 190)AAH29822

This protein carries no "tag".

The protein has a calculated MW of 25 kDa. The protein migrates as 15 kDa under reducing (R) condition and 28 kDa under non-reducing (NR) condition on SDS-PAGE gel.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>90% as determined by reducing (R) and non-reducing (NR) SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in Acetonitrile and TFA. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

ActiveMax® Human PDGF-BB (Catalog # PDB-H4219) SDS-PAGE gel

ActiveMax® Human PDGF-BB on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

Bioactivity

ActiveMax® Human PDGF-BB (Catalog # PDB-H4219) ELISA bioactivity

Immobilized ActiveMax® Human PDGF-BB (Catalog # PDB-H4219) at 5 µg/mL (100 µl/well) can bind Human PDGF R beta, Fc Tag (Catalog # PDB-H5252) with a linear range of 0.02-0.6 µg/mL.

ActiveMax® Human PDGF-BB (Catalog # PDB-H4219) ELISA bioactivity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 0.5-20 ng/mL.

 

Citations

Background

PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.