ActiveMax® Human PDGF-BB (PDB-H4219) is expressed from human 293 cells (HEK293). It contains AA Ser 82 - Thr 190 (Accession # AAH29822).
This protein carries no "tag".
The protein has a calculated MW of 25 kDa. The protein migrates as 15 kDa under reducing (R) condition and 28 kDa under non-reducing (NR) condition on SDS-PAGE gel.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by reducing (R) and non-reducing (NR) SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Acetonitrile and TFA. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human PDGF-BB on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized ActiveMax® Human PDGF-BB (Catalog # PDB-H4219) at 5 µg/mL (100 µl/well) can bind Human PDGF R beta, Fc Tag (Catalog # PDB-H5252) with a linear range of 0.02-0.6 µg/mL (QC tested).
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 0.5-20 ng/mL (routinely tested).
Authors: Chen Z et al.
Journal: EXPERIMENTAL AND THERAPEUTIC MEDICINE 2014
Application: Cell Culture
PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.
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