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Human IGF-II Protein, Fc Tag  pdf  pdf  pdf


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IG2-H4260-100ug
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Synonym

IGF2,C11orf43,FLJ22066,FLJ44734,IGF-II,PP9974

Source

Human IGF-II Protein, Fc Tag, Ala 25 - Glu 91 (Accession # P01344-1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Fc (Thr 106–Lys 330)P01857
IGF-II (Ala 25-Glu 91)P01344-1

Human IGF-II, Fc Tag is fused with a human IgG1 Fc tag at the N-terminus, and has a calculated MW of 35 kDa. The predicted N-terminus is Thr. The reducing (R) protein migrates as 36 kDa in SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

Human IGF-II, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized Human IGF-I R, His Tag (Cat# IGR-H5229) at 2 μg/mL (100 µl/well),can bind Human IGF-II, Fc Tag (Cat# IG2-H4260) with a linear range of 0.06-1 µg/mL (QC tested).

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Background

Insulin-like growth factor 2 (IGF-2) is also known as Somatomedin-A, IGF-II, PP9974, and is one of three protein hormones that share structural similarity to insulin. IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor (also called the cation-independent mannose 6-phosphate receptor), which acts as a signalling antagonist; that is, to prevent IGF2 responses. The major role of IGF2 is as a growth promoting hormone during gestation. In the process of Folliculogenesis, IGF2 is created by Theca cells to act in an autocrine manner on the theca cells themselves, and in a paracrine manner on Granulosa cells in the ovary. IGF2 promotes granulosa cell proliferation during the follicular phase of the menstrual cycle, acting alongside Follicle Stimulating Hormone (FSH). After ovulation has occurred, IGF-2 promotes progesterone secretion during the luteal phase of the menstrual cycle together with Luteinizing Hormone (LH). Thus, IGF2 acts as a Co-hormone together with both FSH and LH. IGF-2 may play a key role in memory and could potentially be used to treat Alzheimer's Disease. It is sometimes produced in excess in islet cell tumours, causing hypoglycemia. Doege-Potter syndrome is a paraneoplastic syndrome in which hypoglycemia is associated with the presence of one or more non-islet fibrous tumors in the pleural cavity. has been shown to interact with IGFBP3 and Transferrin.

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References