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Human RAGE / AGER Protein, Fc Tag  pdf  pdf  pdf


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AGR-H5253-100ug
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AGR-H5253-1mg (500ug x 2)
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Synonym

AGER,RAGE

Source

Human RAGE, Fc Tag (AGR-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ala 344 (Accession # NP_001127).

Predicted N-terminus: Ala 23

Molecular Characterization

RAGE(Ala 23 - Ala 344)NP_001127
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 60.8 kDa. The protein migrates as 80 - 90 kDa and 35 kDa Fc fragment under reducing (R) condition (SDS-PAGE) due to glycosylation and auto-cleavage.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>60% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human RAGE, Fc Tag (Cat. No. AGR-H5253) SDS-PAGE gel

Human RAGE, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 60%.

Bioactivity

Human RAGE, Fc Tag (Cat. No. AGR-H5253) ELISA bioactivity

Immobilized Human RAGE, Fc Tag (Cat. No. AGR-H5253) at 20 μg/mL (100 μL/well) can bind Human HMGB1, His Tag (Cat. No. HM1-H5220) with a linear range of 0.313-5 μg/mL (QC tested).

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Citations

 

Background

Advanced glycosylation end product-specific receptor (AGER) is also known as RAGE, and is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. AGER is highly expressed in endothelial cells. AGER mediates interactions of advanced glycosylation end products (AGE), and also accumulates in vascular tissue in aging and at an accelerated rate in diabetes, as well as acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGER / RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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