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Human Arginase 1 / ARG1 Protein  pdf  pdf  pdf


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Synonym

ARG1,Arginase-1

Source

Human Arginase 1 / ARG1 Protein (Human Arginase 1, His Tag) Met 1 - Lys 322 (Accession # NP_000036) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human Arginase 1, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 35.6 kDa. The predicted N-terminus is Met 1. The reducing (R) protein migrates as 36 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE


Human Arginase 1 / ARG1 Protein
Human Arginase 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
 
 

Background

Arginase-1 (ARG1) is also known as Liver-type arginase, Type I arginase, which belongs to the arginase family. Arginase-1 / ARG1 is a manganese-containing enzyme. The reaction catalyzed by this enzyme is: arginine + H2O → ornithine + urea. It is the final enzyme of the urea cycle. Defects in Arginase-1 / ARG1 are the cause of argininemia (ARGIN).

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References

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