Human Arginase 1 / ARG1 Protein (Human Arginase 1, His Tag) Met 1 - Lys 322 (Accession # NP_000036) was produced in human 293 cells (HEK293) at ACROBiosystems.
Human Arginase 1, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 35.6 kDa. The predicted N-terminus is Met 1. The reducing (R) protein migrates as 36 kDa in SDS-PAGE .
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
Arginase-1 (ARG1) is also known as Liver-type arginase, Type I arginase, which belongs to the arginase family. Arginase-1 / ARG1 is a manganese-containing enzyme. The reaction catalyzed by this enzyme is: arginine + H2O → ornithine + urea. It is the final enzyme of the urea cycle. Defects in Arginase-1 / ARG1 are the cause of argininemia (ARGIN).
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