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Human PLA2G1B Protein  pdf  pdf  pdf


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Synonym

PLA2G1B, Phospholipase A2, PLA2, PLA2A, PPLA2

Source

Recombinant Human PLA2G1B /PLA2 Protein (rh PLA2G1B / PLA2) Ala 23 - Ser 148 (Accession # AAI06726) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

rh PLA2G1B / PLA2 is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 15 kDa. The predicted N-terminus is Ala 23. DTT-reduced Protein migrates as 19 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh PLA2G1B / PLA2 by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Sodium Acetate pH4.5 with 100 mM NaCl. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product format or formulation.

Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


 Recombinant Human PLA2G1B /PLA2 Protein
The purity of rh PLA2G1B / PLA2 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Phosphatidylcholine 2-acylhydrolase 1B (PLA2G1B) is also known as Phospholipase A2 (PLA2), PLA2A, PPLA2, which belongs to the phospholipase A2 family. PLA2G1B is activated by trypsin cleavage in the duodenum and can also be activated by thrombin or autocatalytically. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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