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Human Kininogen 1 / KNG1 Protein  pdf  pdf  pdf


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Synonym

KNG1,Kininogen-1,HMWK,BDK,KNG

Source

Human Kininogen 1 / KNG1 (LMW) Protein (Human Kininogen 1, His Tag) Gln 19 - Ser 427 (Accession # NP_000884) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human Kininogen 1, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 46.6 kDa. The predicted N-terminus is Gln 19. DTT-reduced Protein migrates as 50-68 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Human Kininogen 1, His Tag by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 100 mM Sodium Acetate, 100 mM NaCl, pH 5.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Human Kininogen 1 / KNG1 (LMW) Protein
The purity of Human Kininogen 1, His Tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Kininogen-1 (KNG1) is also known as HMWK, BDK, KNG, is a secreted protein which contains three cystatin domains. KNG1 can be cleaved into the following six chains: Kininogen-1 heavy chain, T-kinin, Bradykinin, Lysyl-bradykinin, Kininogen-1 light chain, Low molecular weight growth-promoting factor. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes; LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

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References

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