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Human Glypican 3 / GPC3 Protein, low endotoxin  pdf  pdf  pdf


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GP3-H5223-100ug
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GP3-H5223-1mg
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Synonym

GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

Source

Human Glypican 3, His Tag (GP3-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # NP_004475).

Predicted N-terminus: Gln25, Ser359 or Val483

Molecular Characterization

Glypican 3(Gln 25 - His 559)NP_004475
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 61.7 kDa. The protein migrates as 40 kDa and 60-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and associated via disulfide bonds.

Endotoxin

Less than 0.1 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Glypican 3, His Tag (Cat. No. GP3-H5223) SDS-PAGE gel

Human Glypican 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Human Glypican 3, His Tag (Cat. No. GP3-H5223) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 0.5 μg/mL (100 μL/well) can bind Human Glypican 3, His Tag (Cat. No. GP3-H5223) with a linear range of 3-128 ng/mL (Routinely tested).

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Background

Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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