Recombinant Influenza A [A/Thailand/1(KAN-1)/2004 (H5N1)] Neuraminidase (NA) (Influenza A [A/Thailand/1(KAN-1)/2004 (H5N1)] NA) His 36 - Lys 449 (Accession # H8PF47) was produced in human 293 cells (HEK293) at ACROBiosystems.
Influenza A [A/Thailand/1(KAN-1)/2004 (H5N1)] NA is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 46.1 kDa. The predicted N-terminus is His 36. The reducing (R) protein migrates as 48 kDa in SDS-PAGE .
Less than 1.0 EU per μg by the LAL method.
>92% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.
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