Our mail server is under upgrade and may miss some of your emails. Please call +1 800-810-0816 to confirm when you get bounce notification or no responses from us.

Browse Products By Molecule:

Products --> Other Recombinant Proteins

Human MAD2L1 Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
MA1-H5123-100ug
    $315.00
MA1-H5123-1mg
    $1500.00
In-Stock
Place Purchase Orders


Synonym

MAD2L1, MAD2, HsMAD2

Source

Recombinant Human MAD2L1 /MAD2 Protein (rh MAD2L1 /MAD2) Met 1 - Asp 205 (Accession # NP_002349) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh MAD2L1 /MAD2 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 24.3 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 24 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh MAD2L1 /MAD2 by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human MAD2L1 /MAD2 Protein
The purity of rh MAD2L1 /MAD2 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Mitotic arrest deficient 2-like protein 1 (MAD2L1) is also known as Mitotic spindle assembly checkpoint protein MAD2A (HsMAD2), MAD2. MAD2L1 / MAD2 belongs to the MAD2 family. MAD2L1 / MAD2 contains one HORMA domain. MAD2L1 is the component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 / MAD2 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References

Related Products