MAD2L1, MAD2, HsMAD2
Recombinant Human MAD2L1 /MAD2 Protein (rh MAD2L1 /MAD2) Met 1 - Asp 205 (Accession # NP_002349) was produced in E.coli cells at ACROBiosystems.
rh MAD2L1 /MAD2 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 24.3 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 24 kDa in SDS-PAGE .
Less than 1.0 EU per μg of the rh MAD2L1 /MAD2 by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Mitotic arrest deficient 2-like protein 1 (MAD2L1) is also known as Mitotic spindle assembly checkpoint protein MAD2A (HsMAD2), MAD2. MAD2L1 / MAD2 belongs to the MAD2 family. MAD2L1 / MAD2 contains one HORMA domain. MAD2L1 is the component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 / MAD2 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
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