Recombinant Human Fumarase /FH Protein (rh Fumarase /FH) Ala 45 - Lys 510 (Accession # AAH03108) was produced in E.coli cells at ACROBiosystems.
rh Fumarase /FH is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 51 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 47 kDa in SDS-PAGE .
Less than 1.0 EU per μg of the rh Fumarase /FH by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Fumarate hydratase, mitochondrial is also known as Fumarase or FH, which belongs to the class-II fumarase/aspartase family and Fumarase subfamily. Fumarase is a homotetramer protein which is expressed in red blood cells. There are two substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors. Fumarase also acts as a tumor suppressor.
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