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Human Fumarase (FH) Protein  pdf  pdf  pdf


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FUE-H5125-100ug
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FUE-H5125-1mg
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Synonym

Fumarase,FH

Source

Human Fumarase, His Tag (FUE-H5125) is expressed from E.coli cells. It contains AA Ala 45 - Lys 510 (Accession # AAH03108).

Predicted N-terminus: Met

Molecular Characterization

Fumarase(Ala 45 - Lys 510)AAH03108
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 51 kDa. The protein migrates as 47 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Fumarase, His Tag (Cat. No. FUE-H5125) SDS-PAGE gel

Human Fumarase, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Fumarate hydratase, mitochondrial is also known as Fumarase or FH, which belongs to the class-II fumarase/aspartase family and Fumarase subfamily. Fumarase is a homotetramer protein which is expressed in red blood cells. There are two substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors. Fumarase also acts as a tumor suppressor.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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