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Human YWHAB Protein, GST Tag  pdf  pdf  pdf


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Synonym

YWHAB, KCIP-1, 14-3-3 beta, GW128, HS1

Source

Recombinant Human YWHAB /14-3-3 Beta Protein, N-GST Tag (rh YWHAB / KCIP-1, GST Tag) Met 1 - Asn 246 (Accession # NP_647539) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh YWHAB / KCIP-1, GST Tag is fused with a GST tag at the N-terminus, and has a calculated MW of 55 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 53 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh YWHAB / KCIP-1, GST Tag by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human YWHAB /14-3-3 Beta Protein, N-GST Tag
The purity of rh YWHAB / KCIP-1, GST Tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB) is also known as 14-3-3 protein beta, GW128, HS1. YWHAB belongs to the 14-3-3 family. YWHAB binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. YWHAB is negative regulator of osteogenesis. YWHAB blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.

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