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Human YWHAB Protein, GST Tag  pdf  pdf  pdf


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YWB-H513a-100ug
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Synonym

YWHAB,KCIP-1,14-3-3 beta,GW128,HS1

Source

Human YWHAB, GST Tag (YWB-H513a) is expressed from E.coli cells. It contains AA Met 1 - Asn 246 (Accession # NP_647539).

Predicted N-terminus: Met 1

Molecular Characterization

GST
YWHAB(Met 1 - Asn 246)NP_647539

This protein carries a GST tag at the N-terminus.

The protein has a calculated MW of 55 kDa. The protein migrates as 53 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human YWHAB, GST Tag (Cat. No. YWB-H513a) SDS-PAGE gel

Human YWHAB, GST Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB) is also known as 14-3-3 protein beta, GW128, HS1. YWHAB belongs to the 14-3-3 family. YWHAB binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. YWHAB is negative regulator of osteogenesis. YWHAB blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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