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Mouse Fc gamma RI / CD64 Protein  pdf  pdf  pdf


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Synonym

FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI

Source

Mouse Fc gamma RI, His Tag (CD4-M5227) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 297 (Accession # NP_034316).

Predicted N-terminus: Glu 25

Molecular Characterization

CD64(Glu 25 - Pro 297)NP_034316
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 31.2 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Mouse Fc gamma RI, His Tag (Catalog # CD4-M5227) SDS-PAGE gel

Mouse Fc gamma RI, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Mouse Fc gamma RI, His Tag (Catalog # CD4-M5227) ELISA bioactivity

(1)Immobilized OKT3 mAb (mouse IgG2a) at 2 μg/mL (100 μl/well) can bind Mouse Fc gamma RI, His Tag (Cat# CD4-M5227) with a linear range of 0.4-3 ng/mL.
(2)The bioactivity is measured by its binding ability to mouse IgG2a in a SPR assay.
Immobilized Mouse Fc gamma RI / CD64 Protein, His Tag (Cat# CD4-M5227) can bind mouse IgG2a with affinity constant of around 6.1 nM (routinely tested).

 

Background

Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.