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Human IgG3 Fc Protein, Tag Free  pdf  pdf  pdf


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IG3-H5200-200ug
    $260.00
IG3-H5200-1mg
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Synonym

IgG Fc

Source

Human IgG3 Fc, Tag Free (IG3-H5200) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 377 (Accession # P01860).

Predicted N-terminus: Glu 99

Molecular Characterization

IgG Fc(Glu 99 - Lys 377)P01860

This protein carries no "tag".

The protein has a calculated MW of 31.2 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) SDS-PAGE gel

Human IgG3 Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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