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Human Insulin R / CD220 (aa 28-944) Protein  pdf  pdf  pdf


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Synonym

INSR,Insulin receptor,IR,CD220

Source

Human Insulin R / CD220 Protein (Human Insulin R (aa 28-944), His Tag) His 28 - Lys 944 (Accession # NP_001073285) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human Insulin R (aa 28-944), His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 86.8 kDa (alpha subunit) & 23 kDa (beta subunit). The predicted N-terminus is His 28 (alpha subunit) & Ser 763 (beta subunit). DTT-reduced Protein migrates as 120-135 kDa (alpha subunit) & 35-46 kDa (beta subunit) in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Human Insulin R (aa 28-944), His Tag by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Human Insulin R / CD220 Protein
The purity of Human Insulin R (aa 28-944), His Tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.

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References

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