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Human IL-12B / NKSF2 / p40 Protein  pdf  pdf  pdf


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Synonym

IL12B,CLMF,CLMF2,NKSF,NKSF2,p40

Source

Human IL-12B, His Tag (NK2-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1).

Predicted N-terminus: Ile 23

Molecular Characterization

IL-12B(Ile 23 - Ser 328)P29460-1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.6 kDa. The protein migrates as 44 kDa and 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human IL-12B, His Tag (Cat. No. NK2-H52H7) SDS-PAGE gel

Human IL-12B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human IL-12B, His Tag (Cat. No. NK2-H52H7) ELISA bioactivity

Immobilized Anti IL23A & IL12B MAb at 2 μg/mL (100 μL/well) can bind Human IL-12B, His Tag (Cat. No. NK2-H52H7) with a linear range of 0.4-13 ng/mL (QC tested).

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Background

Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit beta (IL12B) also known as NKSF2, CLMF2 and P40. Interleukin-12 subunit beta has been shown to interact with IL23. A large excess of monomeric IL12B is also secreted by the cells producing IL12, and exhibits no demonstrable biological activity. Overexpression of IL12B gene has been shown to be associated with the pathogenesis of multiple sclerosis. In addition, studies have revealed that the promoter polymorphism of this gene is implicated in the severity of atopic and non-atopic asthma in children.

References

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