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Human Activin RIIA / ACVR2A Protein, Fc Tag  pdf  pdf  pdf


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Synonym

Activin receptor type IIA,ACTRIIA

Source

Human Activin RIIA, Fc Tag (ACA-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 134 (Accession # NP_001607).

Predicted N-terminus: Ala 20

Molecular Characterization

ACVR2A(Ala 20 - Pro 134)NP_001607
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 40 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Activin RIIA, Fc Tag (Catalog # ACA-H5253) SDS-PAGE gel

Human Activin RIIA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human Activin RIIA, Fc Tag (Catalog # ACA-H5253) ELISA bioactivity

Immobilized Human Activin A, Tag Free (Catalog # ACA-H421b) at 10μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Catalog # ACA-H5253) with a linear range of 0.08-0.625 μg/mL.

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Background

Activin receptor type-2A (ACVR2A) is also known as Activin receptor type IIA, ACTR-IIA, ACTRIIA and ACVR2,which is single-pass type I membrane protein. ACVR2A belongs to the protein kinase superfamily,TKL Ser/Thr protein kinase family and TGFB receptor subfamily.On ligand binding,ACVR2A can forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators.ACVR2A is Receptor for activin A, activin B and inhibin A as well. Several type I I activin receptors have been identified and bind to different activins with different affinities.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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