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Human IL-12B / NKSF2 / p40 Protein, Fc Tag  pdf  pdf  pdf


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Synonym

IL12B, CLMF, CLMF2, NKSF, NKSF2, p40

Source

Recombinant Human IL12B /P40 Protein,C-Fc Tag,HEK293 expressed (rh IL12B /P40 Fc Chimera) Ile 23 - Ser 328 (Accession # AAH67499) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

rh IL12B /P40 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 61.3 kDa. The predicted N-terminus is Ile 23. DTT-reduced Protein migrates as 70-80 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh IL12B /P40 Fc Chimera by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human IL12B /P40 Protein,C-Fc Tag,HEK293 expressed
The purity of rh IL12B /P40 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit beta (IL12B) also known as NKSF2, CLMF2 and P40. Interleukin-12 subunit beta has been shown to interact with IL23. A large excess of monomeric IL12B is also secreted by the cells producing IL12, and exhibits no demonstrable biological activity. Overexpression of IL12B gene has been shown to be associated with the pathogenesis of multiple sclerosis. In addition, studies have revealed that the promoter polymorphism of this gene is implicated in the severity of atopic and non-atopic asthma in children.

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References