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Human VEGF165 Protein, His Tag  pdf  pdf  pdf


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Synonym

RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

Source

Human VEGF165 Protein, His Tag (VE5-H5248) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).

Predicted N-terminus: His

Molecular Characterization

Poly-his
VEGF165(Ala 27 - Arg 191)P15692-4

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 20 kDa (monomer). As a result of glycosylation, The protein migrates as 25 kDa (monomer) under reducing (R) condition and 43-50 kDa (homodimer) under non-reducing (NR) condition on SDS-PAGE gel.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human VEGF165 Protein, His Tag (Catalog # VE5-H5248) SDS-PAGE gel

Human VEGF165 Protein, His Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human VEGF165 Protein, His Tag (Catalog # VE5-H5248) ELISA bioactivity

Immobilized Human VEGF165 Protein, His Tag (Catalog # VE5-H5248) at 2 μg/mL can bind VEGFR1/R2-Fc with a linear range of 0.1-3 ng/mL (QC tested).

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Background

VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.