IL12A, CLMF, IL-12A, NFSK, NKSF1, P35
Recombinant Human IL12A /NKSF1 Protein,C-Fc Tag,HEK293 expressed (rh IL12A / NKSF1 Fc Chimera) Arg 23 - Ser 219 (Accession # P29459) was produced in human 293 cells (HEK293) at ACROBiosystems.
rh IL12A / NKSF1 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 49.2 kDa. The predicted N-terminus is Arg 23. DTT-reduced Protein migrates as 65-80 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the rh IL12A / NKSF1 Fc Chimera by the LAL method.
>92% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product form or formulation.
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit alpha (IL12A) also known as NKSF1, CLMF1 and P35, IL12A shows significant sequence similarity to IL-6, G-CSF, and exerts biological activities only when the IL12B is co-expressed. IL12B deficient mice are resistant to the induction of experimental chronic inflammatory diseases whereas IL12A knock-out mice develop more severe forms, suggesting opposite functions of the two subunits in the outcome of chronic inflammatory diseases.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.