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Human IL-12A / NKSF1 / p35 Protein, Fc Tag  pdf  pdf  pdf


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Synonym

IL12A, CLMF, IL-12A, NFSK, NKSF1, P35

Source

Recombinant Human IL12A /NKSF1 Protein,C-Fc Tag,HEK293 expressed (rh IL12A / NKSF1 Fc Chimera) Arg 23 - Ser 219 (Accession # P29459) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

rh IL12A / NKSF1 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 49.2 kDa. The predicted N-terminus is Arg 23. DTT-reduced Protein migrates as 65-80 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh IL12A / NKSF1 Fc Chimera by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human  IL12A /NKSF1 Protein,C-Fc Tag,HEK293 expressed
The purity of rh IL12A / NKSF1 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit alpha (IL12A) also known as NKSF1, CLMF1 and P35, IL12A shows significant sequence similarity to IL-6, G-CSF, and exerts biological activities only when the IL12B is co-expressed. IL12B deficient mice are resistant to the induction of experimental chronic inflammatory diseases whereas IL12A knock-out mice develop more severe forms, suggesting opposite functions of the two subunits in the outcome of chronic inflammatory diseases.

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References