Recombinant Human AKT1 /PKB Protein,C-His & C-Twin Strep Tag (rh AKT1 / PKB,His.Twin Strep) Met 1 - Ala 480 (Accession # AAH00479) was produced in human 293 cells (HEK293) at ACROBiosystems.
rh AKT1 / PKB,His.Twin Strep is fused with Twin Strep tagged polyhistidine at the C-terminus, and has a calculated MW of 59.5 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 60-66 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the rh AKT1 / PKB,His.Twin Strep by the LAL method.
>92% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 20 mM tris, 150 mM NaCl, pH8.0 . Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
RAC-alpha serine/threonine-protein kinase (AKT1) is also known PKB, Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt and RAC-PK-alpha, which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily and is expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. AKT also regulates the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity.
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