Human Cathepsin E, His Tag (CTE-H5225) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Pro 396 (pro & mature) & Ile 54 - Pro 396 (mature) (Accession # P14091-1).
Predicted N-terminus: Ser 20 (pro & mature) or Ile 54 (mature)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 41.6 kDa. The protein migrates as 40-46 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Cathepsin E, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Cathepsin E (CTSE) is also known as Cd152,Ly56 and Ctla4,which can be cleaved into the following 2 chains: Cathepsin E form I and Cathepsin E form II. Cathepsin E belongs to the peptidase A1 family. CTSE is similar to cathepsin D, but slightly broader specificity. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome. CTSE is expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen, but not expressed in resting B-lymphocytes. CTSE may have a role in immune function,activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.At the same time, CTSE is also probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation.
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