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Human IL-12B & IL-12A Heterodimer Protein, His Tag & Flag Tag  pdf  pdf  pdf


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Synonym

IL12,p70,Interleukin-12

Source

Recombinant Human IL-12B & IL-12A heterodimer protein (Human IL-12B & IL-12A heterodimer) Ile 23 - Ser 328 (IL-12B, AAH67499) and Arg 23 - Ser 219 (IL-12A, P29459) was produced in human 293 cells (HEK293) at ACROBiosystems.

Predicted N-terminus: Ile 23 (IL12B) and Arg 23 (IL12A)

Molecular Characterization

IL-12B (Ile 23 - Ser 328)AAH67499
His
IL-12A (Arg 23 - Ser 219)P29459
Flag

The protein contains two subunits. Subunit IL-12B is fused with his tag at the C-terminus and subunit IL-12A is fused with flag tag at the C-terminus.

The protein has a calculated MW of 36.6 kDa (IL12B) and 23.8 kDa (IL12A). The protein migrates as 33-47 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human IL-12B & IL-12A, His Tag & Flag Tag (Catalog # IL2-H4210) SDS-PAGE gel

Human IL-12B & IL-12A, His Tag & Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.