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Human FAM171B Protein, Fc Tag  pdf  pdf  pdf


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Synonym

FAM171B,KIAA1946,NPD019

Source

Recombinant Human FAM171B /KIAA1946 Protein,C-Fc Tag (rh FAM171B /KIAA1946 Fc Chimera) Glu 33 - Thr 355 (Accession # AAH60872) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

rh FAM171B /KIAA1946 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 62.7 kDa. The predicted N-terminus is Glu 33. The reducing (R) protein migrates as 80-95 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh FAM171B /KIAA1946 Fc Chimera by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human FAM171B /KIAA1946 Protein,C-Fc Tag
The purity of rh FAM171B /KIAA1946 Fc Chimera was determined by SDS-PAGE under reducing (R) condition and staining overnight with Coomassie Blue.
 
 

Background

Family with sequence similarity 171, member B (FAM171B) is also known as KIAA1946, NPD019, which is a single-pass type I membrane protein, belongs to the FAM171 family.

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References

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