Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Other Recombinant Proteins

Human IDH1 Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
ID1-H5120-50ug
    $250.00
ID1-H5120-1mg
    $1600.00
In-stock
Place Purchase Orders


Synonym

IDH1,PICD,IDP

Source

Human IDH1, His Tag (ID1-H5120) is expressed from E.coli cells. It contains AA Met 1 - Leu 414 (Accession # NP_005887).

Predicted N-terminus: Met 1

Molecular Characterization

IDH1(Met 1 - Leu 414)NP_005887
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.5 kDa. The protein migrates as 47.5 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 0.05% Brij-35, 1 mM DTT, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human IDH1, His Tag (Cat. No. ID1-H5120) SDS-PAGE gel

Human IDH1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Isocitrate dehydrogenase 1 (IDH1) is also known as Cytosolic NADP-isocitrate dehydrogenase (NADP(+)-specific ICDH), Oxalosuccinate decarboxylase and PICD, which is one of the Isocitrate dehydrogenases that can catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD+ as the electron acceptor and the other NADP+. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Related Products

Questions & Comments