Recombinant Human IDH1 /PICD Protein (rh IDH1) Met 1 - Leu 414 (Accession # NP_005887) was produced in E.coli cells at ACROBiosystems.
rh IDH1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.5 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 47.5 kDa in SDS-PAGE .
Less than 1.0 EU per μg of the rh IDH1 by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,0.05% Brij-35,1 mM DTT, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Isocitrate dehydrogenase 1 (IDH1) is also known as Cytosolic NADP-isocitrate dehydrogenase (NADP(+)-specific ICDH), Oxalosuccinate decarboxylase and PICD, which is one of the Isocitrate dehydrogenases that can catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD+ as the electron acceptor and the other NADP+. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
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