MABSol® Biotinylated Human FGF basic (BFF-H8117) is expressed from E.coli cells. It contains AA Pro 143 - Ser 288 (Accession # NP_001997). It is the biotinylated form of Human FGF basic Protein, Tag Free (Cat # BFF-H4117).
Predicted N-terminus: Met
The product does not contain any epitope tags.
The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
The biotin to protein ratio is 4.5-5.5 as determined by the HABA assay.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human FGF basic on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. bFGF is a critical component of human embryonic stem cell culture medium. bFGF is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.
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