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Human Thioredoxin-2 / TXN2 Protein  pdf  pdf  pdf


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Synonym

TXN2,Thioredoxin-2,MTRX,TRX2,mitochondrial

Source

Recombinant Human TXN2 /Thioredoxin-2 Protein (rh TXN2 / Thioredoxin-2) Thr 60 - Gly 166 (Accession # AAH13726) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh TXN2 / Thioredoxin-2 is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 12.8 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 13 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh TXN2 / Thioredoxin-2 by the LAL method.

Purity

>97% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl,pH8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human TXN2 /Thioredoxin-2 Protein
The purity of rh TXN2 / Thioredoxin-2 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Thioredoxin-2 (TXN2),a member of the thioredoxin family,is also known as mitochondrial (MTRX or Mt-Trx) and TRX2.TXN2 have two important domains: Redox-active center and Transit peptide. TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Also, TXN2 could be involved in the resistance to anti-tumor agents. TNX2 possesses a dithiol-reducing activity as well.

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References

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