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Human Thioredoxin-2 / TXN2 Protein  pdf  pdf  pdf


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Synonym

TXN2,Thioredoxin-2,MTRX,TRX2,mitochondrial

Source

Human Thioredoxin-2, His Tag (TX2-H5141) is expressed from E.coli cells. It contains AA Thr 60 - Gly 166 (Accession # AAH13726).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
TXN2(Thr 60 - Gly 166)AAH13726

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>97% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Thioredoxin-2, His Tag (Cat. No. TX2-H5141) SDS-PAGE gel

Human Thioredoxin-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

 

Background

Thioredoxin-2 (TXN2),a member of the thioredoxin family,is also known as mitochondrial (MTRX or Mt-Trx) and TRX2.TXN2 have two important domains: Redox-active center and Transit peptide. TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Also, TXN2 could be involved in the resistance to anti-tumor agents. TNX2 possesses a dithiol-reducing activity as well.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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