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Human TPK1 Protein  pdf  pdf  pdf


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Synonym

TPK1,PP20,hTPK1

Source

Recombinant Human TPK1 Protein (rh TPK1) Glu 2 - Ser 243 (Accession # AAH68460) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh TPK1 is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 28 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 28 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh TPK1 by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl,pH8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human TPK1 Protein
The purity of rh TPK1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Thiamin pyrophosphokinase 1 (TPK1),a member of the thiamine pyrophosphokinase family, is also known as Thiamine pyrophosphokinase 1, Placental protein 20 (PP20) and hTPK1,which is detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.TPK1 is regarded as a Kinase and a Transferase that can bind ATP and Nucleotide. TPK1 catalyzes the phosphorylation of thiamine to thiamine pyrophosphate and can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. As for disease,TPK1 is relevant to thiamine metabolism dysfunction syndrome 5, episodic encephalopathy type (THMD5).

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References

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