Human Testican 1, His Tag (SP1-H5248) is expressed from human 293 cells (HEK293). It contains AA Arg 22 - Trp 439 (Accession # AAH30691).
Predicted N-terminus: Arg 22
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 47.8 kDa. The protein migrates as 50-60 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>85% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS,pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Testican 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
Testican-1 is also known as protein SPOCK，SPOCK1, which contains one azal-like domain and one thyroglobulin type-1 domain.SPOCK1 is an extracellular heparan/chondroitin sulfate proteoglycan. Members of this family are known as testicans, also called SPOCKs. SPOCKs are enriched in brain and have been shown to regulate neuronal attachment and outgrowth. They contain inhibitory regions in several domains targeted to different classes of protease, and in some cases may act as protease inhibitors. Little is known about SPOCK1's function until now. It may play a role in cell-cell and cell-matrix interactions except for contributing to various neuronal mechanisms in the central nervous system.
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