Recombinant Human FAM3B Protein,C-Fc Tag (rh FAM3B Fc Chimera) Glu 30 - Ser 235 (Accession # NP_478066) was produced in human 293 cells (HEK293) at ACROBiosystems.
rh FAM3B Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 49.5 kDa. The predicted N-terminus is Glu 30. DTT-reduced Protein migrates as 50-65 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the rh FAM3B Fc Chimera by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Protein FAM3B,a cytokine-like protein identified in 2002,is also known as cytokine-like protein 2-21, pancreatic-derived factor (PANDER), C21orf11 and C21orf76,which is highly expressed in the pancreas and also found in the colon, kidney, prostate, small intestine and testis. There are 2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46. FAM3B can induce apoptosis of alpha and beta cells in a dose- and time-dependent manner. Previous studies showed that FAM3B regulates glucose and lipid metabolism through interaction with liver and endocrine pancreas. FAM3B silencing activates both extrinsic and intrinsic apoptotic pathways. In general, silencing FAM3B promoted p53 phosphorylation and induced p53 accumulation by decreasing Mdm2 expression, which resulted in apoptotic cell death.
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