Recombinant Human FETUB /Fetuin-B Protein (rh FETUB / Fetuin-B) Cys 16 - Pro 382 (Accession # AAH69670 ) was produced in human 293 cells (HEK293) at ACROBiosystems.
rh FETUB / Fetuin-B is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 42.4 kDa. The predicted N-terminus is Cys 16. DTT-reduced Protein migrates as 48-60 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the rh FETUB / Fetuin-B by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl,pH8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Fetuin-B (FETUB), a second member of the fetuin family in mammals, is also known as Fetuin-like protein IRL685, 16G2 and Gugu, which is expressed in liver and testis and contains 2 cystatin fetuin-B-type domains. FETUB is a protease inhibitor required for egg fertilization. FETUB is also required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity). As a liver-derived plasma protein, FETUB is essential for fertilization.
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