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Human Latexin / LXN Protein  pdf  pdf  pdf


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Synonym

LXN,Latexin,ECI,TCI

Source

Recombinant Human LXN /Latexin Protein (rh LXN / Latexin) Glu 2 - Glu 222 (Accession # AAH08438) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh LXN / Latexin is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 26.6 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 32 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh LXN / Latexin by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 150 mM Nacl, pH8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human LXN /Latexin  Protein
The purity of rh LXN / Latexin was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Latexin (LXN) ,a member of the protease inhibitor I47 (latexin) family, is also known as endogenous carboxypeptidase inhibitor (ECI), tissue carboxypeptidase inhibitor (TCI) and protein MUM, which is highly expressed in heart, prostate, ovary, kidney, pancreas and colon, moderate or low in other tissues including brain. LXN is hardly reversible, non-competitive, and a potent inhibitor of CPA1, CPA2 and CPA4. LXN may play a role in inflammation as well.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References

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