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Human Latexin / LXN Protein  pdf  pdf  pdf


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LXN-H5140-50ug
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Synonym

LXN,Latexin,ECI,TCI

Source

Human Latexin, His Tag (LXN-H5140) is expressed from E.coli cells. It contains AA Glu 2 - Glu 222 (Accession # AAH08438).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
Latexin(Glu 2 - Glu 222)AAH08438

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 26.6 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Latexin, His Tag (Cat. No. LXN-H5140) SDS-PAGE gel

Human Latexin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Latexin (LXN) ,a member of the protease inhibitor I47 (latexin) family, is also known as endogenous carboxypeptidase inhibitor (ECI), tissue carboxypeptidase inhibitor (TCI) and protein MUM, which is highly expressed in heart, prostate, ovary, kidney, pancreas and colon, moderate or low in other tissues including brain. LXN is hardly reversible, non-competitive, and a potent inhibitor of CPA1, CPA2 and CPA4. LXN may play a role in inflammation as well.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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