Recombinant Human SBDS Protein (rh SBDS) Ser 2 - Glu 250 (Accession # AAH65700) was produced in E.coli cells at ACROBiosystems.
rh SBDS is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 30 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 30-33 kDa in SDS-PAGE .
Less than 1.0 EU per μg of the rh SBDS by the LAL method.
>98% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product form or formulation.
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Shwachman-Bodian-Diamond syndrome protein (SBDS),a member of the SDO1/SBDS family, is also known as ribosome maturation protein SBDS. SBDS is required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFTUD1, SBDS triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby SBDS can activate ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. SBDS is also required for normal levels of protein synthesis. Furthermore, SBDS may play a role in cellular stress resistance.,response to DNA damage and cell proliferation.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.