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Human S100B Protein  pdf  pdf  pdf


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S1B-H5143-100ug
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Synonym

S100B,NEF,S100,S100beta

Source

Human S100B, His Tag (S1B-H5143) is expressed from E.coli cells. It contains AA Met 1 - Glu 92 (Accession # NP_006263).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
S100B(Met 1 - Glu 92)NP_006263

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 11.5 kDa. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human S100B, His Tag (Cat. No. S1B-H5143) SDS-PAGE gel

Human S100B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

S-100 protein beta chain (S100B),a member of the S-100 family, is also known as S-100 protein subunit beta and S100 calcium-binding protein B, which contains two EF-hand domains. S100B can weakly bind calcium but bind zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Also, S100B can bind to and initiate the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction,S100B may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Fuethermore,S100B could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. S100B may mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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