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Human Alpha-endosulfine / ENSA Protein  pdf  pdf  pdf


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ENA-H5140-100ug
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Synonym

ENSA,Alpha-endosulfine,ARPP-19e,endosulfine Alpha

Source

Human Alpha-endosulfine, His Tag (ENA-H5140) is expressed from E.coli cells. It contains AA Ser 2 - Glu 121 (Accession # AAH00436).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
ENSA(Ser 2 - Glu 121)AAH00436

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 14.2 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Alpha-endosulfine, His Tag (Cat. No. ENA-H5140) SDS-PAGE gel

Human Alpha-endosulfine, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

Background

Alpha-endosulfine (ENSA),a member of the endosulfine family, is also known as ARPP-19e,which is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. ENSA is a protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, ENSA is able to specifically interact with PPP2R2D (PR55-delta) and inhibit its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). ENSA acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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