Recombinant Human ENSA /Alpha-endosulfine Protein (rh ENSA /Alpha-endosulfine) Ser 2 - Glu 121 (Accession # AAH00436) was produced in E.coli cells at ACROBiosystems.
rh ENSA /Alpha-endosulfine is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 14.2 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 18 kDa in SDS-PAGE .
Less than 1.0 EU per μg of the rh ENSA /Alpha-endosulfine by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product form or formulation.
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Alpha-endosulfine (ENSA),a member of the endosulfine family, is also known as ARPP-19e,which is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. ENSA is a protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, ENSA is able to specifically interact with PPP2R2D (PR55-delta) and inhibit its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). ENSA acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.