Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Other Recombinant Proteins

Human B3GAT3 Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
B33-H5141-100ug
    $250.00
B33-H5141-1mg (500ug x 2)
    $1300.00
In-stock
Place Purchase Orders


Synonym

B3GAT3,GlcAT-I,GlcUAT-I

Source

Human B3GAT3, His Tag (B33-H5141) is expressed from E.coli cells. It contains AA Glu 72 - Val 335 (Accession # AAH07906).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
B3GAT3(Glu 72 - Val 335)AAH07906

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 30.2 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human B3GAT3, His Tag (Cat. No. B33-H5141) SDS-PAGE gel

Human B3GAT3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

Background

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3), a member of the glycosyltransferase 43 family, is also known as beta-1,3-glucuronyltransferase 3, glucuronosyltransferase I (GlcAT-I) and UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase (GlcUAT-I). B3GAT3 plays a role in glycosaminoglycans biosynthesis and be involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. B3GAT3 can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Furthermore,B3GAT3 shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Related Products

Questions & Comments