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Human B3GAT3 Protein  pdf  pdf  pdf


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Synonym

B3GAT3,GlcAT-I,GlcUAT-I

Source

Recombinant Human B3GAT3 Protein (rh B3GAT3) Glu 72 - Val 335 (Accession # AAH07906) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh B3GAT3 is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 30.2 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 32 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh B3GAT3 by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human B3GAT3 Protein
The purity of rh B3GAT3 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3), a member of the glycosyltransferase 43 family, is also known as beta-1,3-glucuronyltransferase 3, glucuronosyltransferase I (GlcAT-I) and UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase (GlcUAT-I). B3GAT3 plays a role in glycosaminoglycans biosynthesis and be involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. B3GAT3 can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Furthermore,B3GAT3 shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc.

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References

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