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Human UBE2F Protein  pdf  pdf  pdf


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Synonym

UBE2F,NCE2

Source

Recombinant Human UBE2F Protein (rh UBE2F) Met 1 - Arg 185 (Accession # AAH10549) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh UBE2F is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 21.9 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 23-25 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh UBE2F by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human UBE2F Protein
The purity of rh UBE2F was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Ubiquitin-conjugating enzyme E2 F (UBE2F), a member of the ubiquitin-conjugating enzyme family and UBE2F subfamily, is also known as NEDD8-conjugating enzyme UBE2F, NEDD8 carrier protein UBE2F and NEDD8-conjugating enzyme 2 (NCE2),which can interact with UBA3 and RBX2. The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.UBE2F also can accept the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyze its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

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References

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