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Human UBE2F Protein  pdf  pdf  pdf


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Synonym

UBE2F,NCE2

Source

Human UBE2F, His Tag (UBF-H5148) is expressed from E.coli cells. It contains AA Met 1 - Arg 185 (Accession # AAH10549).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
UBE2F(Met 1 - Arg 185)AAH10549

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 21.9 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human UBE2F, His Tag (Cat. No. UBF-H5148) SDS-PAGE gel

Human UBE2F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

Background

Ubiquitin-conjugating enzyme E2 F (UBE2F), a member of the ubiquitin-conjugating enzyme family and UBE2F subfamily, is also known as NEDD8-conjugating enzyme UBE2F, NEDD8 carrier protein UBE2F and NEDD8-conjugating enzyme 2 (NCE2),which can interact with UBA3 and RBX2. The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.UBE2F also can accept the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyze its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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