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Human SUMO1 Protein  pdf  pdf  pdf


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Synonym

SUMO1,SMT3C,UBL1,Sentrin,GMP1

Source

Recombinant Human SUMO1 /SMT3C Protein (rh SUMO1 / SMT3C) Met 1 - Val 101 (Accession # AAH66306) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh SUMO1 / SMT3C is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 12.4 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 20 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh SUMO1 / SMT3C by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human SUMO1 /SMT3C Protein
The purity of rh SUMO1 / SMT3C was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Small ubiquitin-related modifier 1 (SUMO1),a member of the ubiquitin family and SUMO subfamily, is also known as GAP-modifying protein 1 (GMP1), SMT3 homolog 3, ubiquitin-homology domain protein PIC1 and ubiquitin-like protein UBL1,which contains one ubiquitin-like domain. Cleavage of precursor form by SENP1 or SENP2 is necessary for the function of SUMO1. Polymeric SUMO1 chains undergo polyubiquitination by RNF4. SUMO1 may also regulate a network of genes involved in palate development.SUMO1 plays an important role in modulation of NOX activity required for ROS generation.

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References

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