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Human GPD1 / GPD-C Protein  pdf  pdf  pdf


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GP1-H5148-100ug
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Synonym

GPD1,GPD-C,GPDH-C,GPDH

Source

Human GPD1, His Tag (GP1-H5148) is expressed from E.coli cells. It contains AA Met 1 - Met 349 (Accession # AAH32234).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
GPD1(Met 1 - Met 349)AAH32234

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 38.4 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human GPD1, His Tag (Cat. No. GP1-H5148) SDS-PAGE gel

Human GPD1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (GPD1), a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family, is also known as GPD-C or GPDH-C. The function of GPD1 is inhibited by zinc ions and sulfate. GPD1 can also interact selectively and non-covalently with nicotinamide adenine dinucleotide (NAD), a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NAD+, or the reduced form, NADH. Mutations in GPD1 gene are a cause of Hypertriglyceridemia, transient infantile (HTGIT).

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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