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Human Galactowaldenase / GALE Protein  pdf  pdf  pdf


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GAE-H5142-100ug
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Synonym

GALE,UDP-glucose 4-epimerase,Galactowaldenase

Source

Human Galactowaldenase, His Tag (GAE-H5142) is expressed from E.coli cells. It contains AA Met 1 - Ala 348 (Accession # AAH01273).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
GALE(Met 1 - Ala 348)AAH01273

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 39 kDa. The protein migrates as 39 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 20 mM Hepes, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Galactowaldenase, His Tag (Cat. No. GAE-H5142) SDS-PAGE gel

Human Galactowaldenase, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

UDP-glucose 4-epimerase, a member of the NAD(P)-dependent epimerase (dehydratase) family, is also known as galactowaldenase (GALE), UDP-N-acetylgalactosamine 4-epimerase (UDP-GalNAc 4-epimerase) and UDP-N-acetylglucosamine 4-epimerase (UDP-GlcNAc 4-epimerase),which is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. GALE can catalyze two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and GALE (UDP-GalNAc) when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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