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Human UCH-L1 Protein  pdf  pdf  pdf


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Synonym

UCHL1,PGP9.5

Source

Recombinant Human UCHL1 Protein (rh UCHL1) Met 1 - Ala 223 (Accession # NP_004172) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh UCHL1 is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 25.7 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 26 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh UCHL1 by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 20mM Tris, 500mM Nacl,1mM DTT,pH 8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human UCHL1 Protein
The purity of rh UCHL1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ,a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also,UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.

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References

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