Recombinant Human S100A1 Protein (rh S100A1) Met 1 - Ser 94 (Accession # AAH14392) was produced in E.coli cells at ACROBiosystems.
rh S100A1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 11.4 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 11 kDa in SDS-PAGE .
Less than 1.0 EU per μg of the rh S100A1 by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product form or formulation.
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Protein S100-A1 / S100A1 is a Ca2+binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. S100-A1 can weakly bind calcium but bind zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.