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Human S100A1 Protein  pdf  pdf  pdf


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Synonym

S100A1

Source

Recombinant Human S100A1 Protein (rh S100A1) Met 1 - Ser 94 (Accession # AAH14392) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh S100A1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 11.4 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 11 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh S100A1 by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human S100A1 Protein
The purity of rh S100A1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Protein S100-A1 / S100A1 is a Ca2+binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. S100-A1 can weakly bind calcium but bind zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

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References