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Biotinylated Mouse SCF / KITLG, ultra sensitivity (primary amine labeling)  pdf  pdf  pdf


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Synonym

KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL

Source

MABSol® Biotinylated Mouse SCF / KITLG (SCF-M8228) is expressed from human HEK293 cells. It contains AA Lys 26 - Ala 189 (Accession # NP_038626). It is the biotinylated form of Mouse SCF Protein, His Tag (Cat # SCF-M5228).

Predicted N-terminus: Lys 26

Molecular Characterization

SCF(Lys 26 - Ala 189)NP_038626
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 19.1 kDa. The protein migrates as 19-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Biotinylation

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio

The biotin to protein ratio is 0.8-1.5 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>98% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Mouse SCF (Catalog # SCF-M8228) SDS-PAGE gel

Biotinylated Mouse SCF on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity

Biotinylated Mouse SCF (Catalog # SCF-M8228) ELISA bioactivity

Immobilized Human CD117, His Tag (Cat# CD7-H52H4) at 2 μg/mL (100 μl/well) can bind Biotinylated Mouse SCF (Cat# SCF-M8228) with a linear range of 0.8-62 ng/mL.

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Background

Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.