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Biotinylated Human Transferrin R / CD71, ultra sensitivity (primary amine labeling)  pdf  pdf  pdf


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Synonym

CD71,TFRC,TFR,sTfR,TfR1,Trfr,T9,p90

Source

MABSol® Biotinylated Human Transferrin R / CD71 (TFR-H8243) is expressed from human HEK293 cells. It contains AA Cys 89 - Phe 760 (Accession # AAH01188.1). It is the biotinylated form of Human Transferrin R protein, His Tag (Cat. No. CD1-H5243).

Predicted N-terminus: His

Molecular Characterization

Poly-his
TfR(Cys 89 - Phe 760)AAH01188.1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 76 kDa. The protein migrates as 80-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio

The biotin to protein ratio is 1-2 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human Transferrin R (Cat. No. TFR-H8243) SDS-PAGE gel

Biotinylated Human Transferrin R on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity

Biotinylated Human Transferrin R (Cat. No. TFR-H8243) ELISA bioactivity

Immobilized Human Transferrin, His Tag (Cat. No. TRN-H4229) at 10 μg/mL (100 µL/well),can bind Biotinylated Human Transferrin R (Cat. No. TFR-H8243) with a linear range of 2-200 ng/mL (QC tested).

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Background

CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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