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Biotinylated Human GPA33, ultra sensitivity (primary amine labeling)  pdf  pdf  pdf


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Synonym

GPA33,A33

Source

MABSol® Biotinylated Human GPA33 / A33 (GP3-H8224) is expressed from human HEK293 cells. It contains AA Ile 22 - Val 235 (Accession # AAH74830). It is the biotinylated form of Human GPA33 / A33 Protein (Cat # GP3-H5224).

Predicted N-terminus: Ile 22

Molecular Characterization

GPA33(Ile 22 - Val 235)AAH74830
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 24.5 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio

The biotin to protein ratio is 1-2 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human GPA33 (Catalog # GP3-H8224) SDS-PAGE gel

Biotinylated Human GPA33 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.