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Biotinylated Human CD3 epsilon, ultra sensitivity (primary amine labeling)  pdf  pdf  pdf


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Synonym

FLJ18683,T3E,TCRE,CD3E,CD3-epsilon

Source

MABSol® Biotinylated Human CD3 epsilon (CDE-H8223) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1). It is the biotinylated form of Human CD3 epsilon Protein (Cat # CDE-H5223).

Predicted N-terminus: Asp 23

Molecular Characterization

CD3E(Asp 23 - Asp 126)NP_000724.1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 13.2 kDa. The protein migrates as 18-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-2 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>90% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human CD3 epsilon (Catalog # CDE-H8223) SDS-PAGE gel

Biotinylated Human CD3 epsilon on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Biotinylated Human CD3 epsilon (Catalog # CDE-H8223) ELISA bioactivity

Immobilized Anti Human CD3 MAb, mouse IgG1 (Clone # SP34-2) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD3E, His Tag (Catalog # CDE-H8223) with a linear range of 0.05-2 ng/mL.

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Citations

Background

CD3e molecule, epsilon also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.